Analysis of FCP gene expression in dark-adapted diatom cultures revealed that mRNA levels increase 5- to 6-fold in response to white light irradiation and peak around 6 to 8 h. To circumvent this problem, we have developed a high-throughput semi-quantitative RT-PCR-based protocol and used it to monitor expression of a gene encoding a fucoxanthin, chlorophyll a/c-binding protein (FCP) in the centric planktonic diatom Thalassiosira weissflogii. This has seriously hampered studies of the basic biology of such organisms. The low cell densities of diatoms and other phytoplankton in culture has precluded the use of classical RNA analysis techniques for routine studies of gene expression in large numbers of samples. Leblanc, C, Falciatore, A, Watanabe, M, Bowler, CĪctins, Algal Proteins, Amino Acid Sequence, Diatoms, DNA, Gene Expression Regulation, Genes, Light, Light-Harvesting Protein Complexes, Molecular Sequence Data, Photosynthetic Reaction Center Complex Proteins, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Spectrophotometry Semi-quantitative RT-PCR analysis of photoregulated gene expression in marine diatoms.
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